REACTIVATION OF GLYCERINATED CILIA FROM TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
Reactivation of Glycerinated Cilia from Tetrahymena Pyriformis
The ease with which Tetrahymena can be grown in relatively large quantity makes it an exceptionally useful source of cilia for biochemical study. Several methods of isolating the cilia have been described (3, 11). Although excellent for many purposes, these procedures all suffer from the limitation that the isolated cilia seem incapable of being reactivated by ATP. This paper describes a method...
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Cilia isolated from Tetrahymena after ciliary shedding is induced by the addition of CaC1 2 or other salts to cells suspended in an ethanol solution (4, 6, 11) have been studied chemically and enzymatically (4, 6, 1i, 12, 13), but until now have never been shown to be capable of motility in the presence of ATP. In contrast to this, Gibbons (7) has demonstrated the reactivation of glycerolisolat...
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Tetrahymena ciliary membranes were prepared by four different techniques, and their protein composition was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), electron microscopy, and two-dimensional thin-layer peptide mapping. Extraction of the isolated cilia by nonionic detergent solubilized the ciliary membranes but left the axonemal microtubules and dyneine ar...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.25.2.400